Cottonseed Kernel Powder as a Natural Health Supplement: An Approach to Reduce the Gossypol Content and Maximize the Nutritional Benefits

Cottonseed is one of the important by-products cotton crop. Researchers claim that cottonseed with less than 0.45% gossypol quite good for human consumption and animal feeding because it a rich source protein, edible oil, energy. Total free gossypols are influencing parameters reduce nature cottonseed. In present work, multiple quadratic regression models have been prepared to predict reduction in total percent. This response surface method (RSM)-based approach was applied investigate combined effect between input such as acetone level, time extraction, liquid-to-solid ratio (LSR), number extraction cycles, whereas output responses percentage. Analysis Variance (ANOVA) has performed determine highly significant parameter. The optimum combination determined using RSM-based desirability approach, confirmatory experiments were validate combination. Results revealed cycles significantly affects levels. values r-square found above 0.9, which indicates developed suitable reliable predicting